Genotyping and primer details


Genotyping and primer details for SALK_113634c - At3g03500
Line ID SALK_113634c
Locus At3g03500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:833859 of line SALK_113634c Chr3:833859
   
Insertion allele amplicon LBb3 - 29ty
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 29ty: 5'-TATCCTTCTGTTGTTCCTTGCTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:833181
   
wt amplicon (over InsPos) 17uu - 29ty
wt amplicon start Chr3:833181
wt amplicon end Chr3:833961
wt amplicon size 781 bp
Locus specific primer 29ty: 5'-TATCCTTCTGTTGTTCCTTGCTTT-3'
2nd locus specific primer 17uu: 5'-TTTCAAAGTCTTCTTGGCAATTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11