Genotyping and primer details


Genotyping and primer details for SALK_113991c - At1g19680
Line ID SALK_113991c
Locus At1g19680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6806057 of line SALK_113991c Chr1:6806057
   
Insertion allele amplicon LBb3 - 12ds
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 12ds: 5'-AGTACAATTTAATTCGTTTCCCGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6805209
   
wt amplicon (over InsPos) 25dz - 12ds
wt amplicon start Chr1:6805209
wt amplicon end Chr1:6806154
wt amplicon size 946 bp
Locus specific primer 12ds: 5'-AGTACAATTTAATTCGTTTCCCGA-3'
2nd locus specific primer 25dz: 5'-CTGAGAGCCAAGAGTAGCTAAACC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11