Genotyping and primer details


Genotyping and primer details for SALK_114391c - At4g01000
Line ID SALK_114391c
Locus At4g01000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:432924 of line SALK_114391c Chr4:432924
   
Insertion allele amplicon LBb3 - 57vi
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 57vi: 5'-AAATGGAGCTACGCAGAAGTATGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:433260
   
wt amplicon (over InsPos) 58vw - 57vi
wt amplicon start Chr4:432151
wt amplicon end Chr4:433260
wt amplicon size 1110 bp
Locus specific primer 57vi: 5'-AAATGGAGCTACGCAGAAGTATGT-3'
2nd locus specific primer 58vw: 5'-TCAAAAGGGATATTTTTGGAGTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11