Genotyping and primer details


Genotyping and primer details for SALK_114659 - At2g32190
Line ID SALK_114659
Locus At2g32190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:13674710 of line SALK_114659 Chr2:13674710
   
Insertion allele amplicon R204 - SC02
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer SC02: 5'-TTTTGTCAGCATTGTGAGGTTTAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:13673804
   
wt amplicon (over InsPos) 29lt - SC02
wt amplicon start Chr2:13673804
wt amplicon end Chr2:13674996
wt amplicon size 1193 bp
Locus specific primer SC02: 5'-TTTTGTCAGCATTGTGAGGTTTAG-3'
2nd locus specific primer 29lt: 5'-AATAACCGATCCGGTTTGATAGTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11