Genotyping and primer details


Genotyping and primer details for SALK_114715c - At2g45870
Line ID SALK_114715c
Locus At2g45870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:18876731 of line SALK_114715c Chr2:18876731
   
Insertion allele amplicon LBb3 - 19vt
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 19vt: 5'-GATGCCATAAGACTAAGAACCTCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:18877080
   
wt amplicon (over InsPos) 66wi - 19vt
wt amplicon start Chr2:18876133
wt amplicon end Chr2:18877080
wt amplicon size 948 bp
Locus specific primer 19vt: 5'-GATGCCATAAGACTAAGAACCTCG-3'
2nd locus specific primer 66wi: 5'-GGTCTGACGGTATCAAAGAGAGAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11