Genotyping and primer details


Genotyping and primer details for SALK_114735 - At2g24000
Line ID SALK_114735
Locus At2g24000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:10213890 of line SALK_114735 Chr2:10213890
   
Insertion allele amplicon LBb3 - 46fx
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 46fx: 5'-ATGACCCTTGTACCGAAAACTATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:10213289
   
wt amplicon (over InsPos) 13hf - 46fx
wt amplicon start Chr2:10213289
wt amplicon end Chr2:10214350
wt amplicon size 1062 bp
Locus specific primer 46fx: 5'-ATGACCCTTGTACCGAAAACTATG-3'
2nd locus specific primer 13hf: 5'-AAGCCATCATAGACCACATTTAGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11