Genotyping and primer details


Genotyping and primer details for SALK_114838c - At1g51220
Line ID SALK_114838c
Locus At1g51220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:18990276 of line SALK_114838c Chr1:18990276
   
Insertion allele amplicon LBb3 - 84sm
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 84sm: 5'-AAGCTTGCACCACAAGTCTTTAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:18989690
   
wt amplicon (over InsPos) 11tl - 84sm
wt amplicon start Chr1:18989690
wt amplicon end Chr1:18990697
wt amplicon size 1008 bp
Locus specific primer 84sm: 5'-AAGCTTGCACCACAAGTCTTTAAT-3'
2nd locus specific primer 11tl: 5'-GTAACATGTTCCGCATGTGTTAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11