Genotyping and primer details


Genotyping and primer details for SALK_115412 - At1g34065
Line ID SALK_115412
Locus At1g34065 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:12400441 of line SALK_115412 Chr1:12400441
   
Insertion allele amplicon LBb3 - 58vi
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 58vi: 5'-TGCCTTTTAGAGAAGGGTTTATGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:12401056
   
wt amplicon (over InsPos) 69vw - 58vi
wt amplicon start Chr1:12399716
wt amplicon end Chr1:12401056
wt amplicon size 1341 bp
Locus specific primer 58vi: 5'-TGCCTTTTAGAGAAGGGTTTATGA-3'
2nd locus specific primer 69vw: 5'-AGACTTACTTCTGTTGGTACCCGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11