Genotyping and primer details


Genotyping and primer details for SALK_115858c - At3g49660
Line ID SALK_115858c
Locus At3g49660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:18414600 of line SALK_115858c Chr3:18414600
   
Insertion allele amplicon LBb3 - 96ns
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 96ns: 5'-TTCTTTCTTCTGTGTCCAAATCCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:18415220
   
wt amplicon (over InsPos) 10or - 96ns
wt amplicon start Chr3:18414245
wt amplicon end Chr3:18415220
wt amplicon size 976 bp
Locus specific primer 96ns: 5'-TTCTTTCTTCTGTGTCCAAATCCT-3'
2nd locus specific primer 10or: 5'-ACTGGTCATTGTGTGAAAACTCTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11