Genotyping and primer details


Genotyping and primer details for SALK_117783c - At4g21180
Line ID SALK_117783c
Locus At4g21180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:11289900 of line SALK_117783c Chr4:11289900
   
Insertion allele amplicon LBb3 - 27jr
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 27jr: 5'-TGTATAACCCTGCATCAATCACAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:11290380
   
wt amplicon (over InsPos) 17mc - 27jr
wt amplicon start Chr4:11289467
wt amplicon end Chr4:11290380
wt amplicon size 914 bp
Locus specific primer 27jr: 5'-TGTATAACCCTGCATCAATCACAT-3'
2nd locus specific primer 17mc: 5'-GTCAATGTCTTGAGTGTGATCGTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11