Sequences from confirmation amplicon


Confirmation sequences for line SALK_117783c and hit At4g21180 are shown.
Line ID SALK_117783c
Locus At4g21180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11289900
Primers used for PCR 27jr - LBb3, show primer details
   
Sequence Name 74-K113949-SALK_117783C-27jr
GenBank Accession LN486108 [GenBank]
Sequencing primer gene specific primer, 27jr: 5'-TGTATAACCCTGCATCAATCACAT-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K113949-SALK_117783C-27jr
GCAAGCCCATAATATGCTACAAAAACATGAGAATATGGCCTCAATTGTTTCAAACACTCC
TTTAGATTTGCATTTGAAATTGCGAGCACAATTTACAAAGAAGTCTCAATTTTCATTTGA
GGAAAAAACACTATTCTAACATGTAAATGTAAAAACAAACAAACAATGGAGGTTATATAG
TCATTACCTGTCTACCATCCGGATGACCGTACTTCTCAAAGTTTTCGCGGGATAACGGAT
CAGTCAAAGCTTGGTAAGCTTTAGCTATGGACTCCACAAAATATTTATTGGCCTCTGTAC
CATTACAATACACAAATAAGCAATACTAAGAACACATAACAATGTATCAAAAGGGAGGAA
ATAAACGATTATCAAGGAAGAGGACCAGGAAGCATTTCGACCTGGATCAGGATTTTTATC
AGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACG
TTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCC
CTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAG
GCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 63-K113948-SALK_117783C-R210
GenBank Accession LN485824 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K113948-SALK_117783C-R210
TTTGTTTACCCACAATATATCCTGATAAAATCCTGATCCAGGTCGAAATGCTTCCTGGTC
CTCTTCCTTGATAATCGTTTATTTCCTCCCTTTTGATACATTGTTATGTGTTCTTAGTAT
TGCTTATTTGTGTATTGTAATGGTACAGAGGCCAATAAATATTTTGTGGAGTCCATAGCT
AAAGCTTACCAAGCTTTGACTGATCCGTTATCCCGCGAAAACTTTGAGAAGTACGGTCAT
CCGGATGGTAGACAGGTAATGACTATATAACCTCCATTGTTTGTTTGTTTTTACATTTAC
ATGTTAGAATAGTGTTTTTTCCTCAAATGAAAATTGAGACTTCTTTGTAAATTGTGCTCG
CAATTTCAAATGCAAATCTAAAGGAGTGTTTGAAACAATTGAGGCCATATTCTCATGTTT
TTGTAGCATATTATGGGCTTTGCCGTCTCCAAATGATAATTGAAATATTTGTAAATGTGA
TTGATGCAGGNNTTATAC

Last Updated on 06.12.2018 16:11