Genotyping and primer details


Genotyping and primer details for SALK_117958c - At1g03770
Line ID SALK_117958c
Locus At1g03770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:945151 of line SALK_117958c Chr1:945151
   
Insertion allele amplicon LBb3 - 55wm
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 55wm: 5'-GGAAATACAAATAAGCCCACAAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:944656
   
wt amplicon (over InsPos) 04xf - 55wm
wt amplicon start Chr1:944656
wt amplicon end Chr1:945689
wt amplicon size 1034 bp
Locus specific primer 55wm: 5'-GGAAATACAAATAAGCCCACAAGA-3'
2nd locus specific primer 04xf: 5'-CAAGCCTGAACAGTCTACAATGAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11