Genotyping and primer details


Genotyping and primer details for SALK_118107c - At5g53540
Line ID SALK_118107c
Locus At5g53540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:21751177 of line SALK_118107c Chr5:21751177
   
Insertion allele amplicon LBb3 - 19ke
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 19ke: 5'-AAAGCTCAGGTCTTTTCAATGGTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:21750680
   
wt amplicon (over InsPos) 11ls - 19ke
wt amplicon start Chr5:21750680
wt amplicon end Chr5:21751422
wt amplicon size 743 bp
Locus specific primer 19ke: 5'-AAAGCTCAGGTCTTTTCAATGGTA-3'
2nd locus specific primer 11ls: 5'-GAGTCGACTTTTTCCAAATGCTAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11