Genotyping and primer details


Genotyping and primer details for SALK_118939 - At1g69720
Line ID SALK_118939
Locus At1g69720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:26227940 of line SALK_118939 Chr1:26227940
   
Insertion allele amplicon LBb3 - 64vr
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 64vr: 5'-TAAGAGATGGTTGCATGTTGAGTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:26229040
   
Insertion allele amplicon R212 - BI21
T-DNA primer R212: 5'-CCTTAGGCGACTTTTGAACGCGC-3'
Locus specific primer BI21: 5'-AGTAAGTTGGTTTACGACACGCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:26227553
   
wt amplicon (over InsPos) BI20 - BI21
wt amplicon start Chr1:26227553
wt amplicon end Chr1:26228397
wt amplicon size 845 bp
Locus specific primer BI21: 5'-AGTAAGTTGGTTTACGACACGCTT-3'
2nd locus specific primer BI20: 5'-ATCTCCCCTGAAAACTTAAACGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11