Genotyping and primer details


Genotyping and primer details for SALK_119176c - At3g07730
Line ID SALK_119176c
Locus At3g07730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:2469221 of line SALK_119176c Chr3:2469221
   
Insertion allele amplicon LBb3 - 21vt
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 21vt: 5'-GAACGAAGAAAGAGGAAATGTGAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2468773
   
wt amplicon (over InsPos) 65wi - 21vt
wt amplicon start Chr3:2468773
wt amplicon end Chr3:2470050
wt amplicon size 1278 bp
Locus specific primer 21vt: 5'-GAACGAAGAAAGAGGAAATGTGAC-3'
2nd locus specific primer 65wi: 5'-TATGCTGGTTCATAAGGGAATAGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11