Genotyping and primer details


Genotyping and primer details for SALK_119456 - At5g18100
Line ID SALK_119456
Locus At5g18100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:5988412 of line SALK_119456 Chr5:5988412
   
Insertion allele amplicon LBb3 - 21ce
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 21ce: 5'-TGCTTTTACTGGTTTTGTTCTCCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:5987804
   
wt amplicon (over InsPos) 23dw - 21ce
wt amplicon start Chr5:5987804
wt amplicon end Chr5:5988688
wt amplicon size 885 bp
Locus specific primer 21ce: 5'-TGCTTTTACTGGTTTTGTTCTCCT-3'
2nd locus specific primer 23dw: 5'-AGATGATTGAAGTCCGATGATACC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11