Genotyping and primer details

Genotyping and primer details for SALK_119557c - At3g57140
Line ID SALK_119557c
Locus At3g57140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:21150751 of line SALK_119557c Chr3:21150751
Insertion allele amplicon LBb3 - 47kk
Locus specific primer 47kk: 5'-CTTCCTGGAATTGTATAGCTCGTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:21151316
wt amplicon (over InsPos) 15mc - 47kk
wt amplicon start Chr3:21150411
wt amplicon end Chr3:21151316
wt amplicon size 906 bp
Locus specific primer 47kk: 5'-CTTCCTGGAATTGTATAGCTCGTC-3'
2nd locus specific primer 15mc: 5'-CCAACATTTATGGAAGTGTCAATG-3'
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11