Genotyping and primer details


Genotyping and primer details for SALK_119607 - At4g11250
Line ID SALK_119607
Locus At4g11250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:6849806 of line SALK_119607 Chr4:6849806
   
Insertion allele amplicon LBb3 - 22vt
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 22vt: 5'-GTTACCATAAGGGAATTCATGTGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:6850438
   
wt amplicon (over InsPos) 64wi - 22vt
wt amplicon start Chr4:6849373
wt amplicon end Chr4:6850438
wt amplicon size 1066 bp
Locus specific primer 22vt: 5'-GTTACCATAAGGGAATTCATGTGG-3'
2nd locus specific primer 64wi: 5'-GATACTGCTTTTATCTTCTTCACGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11