Genotyping and primer details


Genotyping and primer details for SALK_119825c - At3g08590
Line ID SALK_119825c
Locus At3g08590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:2608812 of line SALK_119825c Chr3:2608812
   
Insertion allele amplicon LBb3 - 37fr
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 37fr: 5'-TTTCAATAAATTTGTGACATGGGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2609219
   
wt amplicon (over InsPos) 16gf - 37fr
wt amplicon start Chr3:2608391
wt amplicon end Chr3:2609219
wt amplicon size 828 bp
Locus specific primer 37fr: 5'-TTTCAATAAATTTGTGACATGGGA-3'
2nd locus specific primer 16gf: 5'-CAACTGCCCTTAAAGTCTCATACC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11