Genotyping and primer details


Genotyping and primer details for SALK_120688c - At5g51680
Line ID SALK_120688c
Locus At5g51680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:20998428 of line SALK_120688c Chr5:20998428
   
Insertion allele amplicon LBb3 - 20ql
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 20ql: 5'-ATTTAGATCTTGAGGTCGAACCAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:20999027
   
Insertion allele amplicon LBb3 - 34db
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 34db: 5'-ACTCAAGGCCTTCTATCATTTTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:20998720
   
Insertion allele amplicon R212 - 55wt
T-DNA primer R212: 5'-CCTTAGGCGACTTTTGAACGCGC-3'
Locus specific primer 55wt: 5'-AAGAAGAATTGGCAACCTTCACTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:20997690
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11