Genotyping and primer details


Genotyping and primer details for SALK_120701c - At1g77420
Line ID SALK_120701c
Locus At1g77420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:29095144 of line SALK_120701c Chr1:29095144
   
Insertion allele amplicon LBb3 - 60vi
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 60vi: 5'-TCAGAGCAATATGGTTCAAATGAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:29095706
   
wt amplicon (over InsPos) 67vw - 60vi
wt amplicon start Chr1:29094576
wt amplicon end Chr1:29095706
wt amplicon size 1131 bp
Locus specific primer 60vi: 5'-TCAGAGCAATATGGTTCAAATGAG-3'
2nd locus specific primer 67vw: 5'-GAGCAGTTGCCTTAAAAATTCATC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11