Genotyping and primer details


Genotyping and primer details for SALK_120726 - At3g07500
Line ID SALK_120726
Locus At3g07500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:2392278 of line SALK_120726 Chr3:2392278
   
Insertion allele amplicon LBb3 - 06vw
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 06vw: 5'-CAAAGCTATGTCTTGTTCACCAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2392886
   
wt amplicon (over InsPos) 20wq - 06vw
wt amplicon start Chr3:2391713
wt amplicon end Chr3:2392886
wt amplicon size 1174 bp
Locus specific primer 06vw: 5'-CAAAGCTATGTCTTGTTCACCAAA-3'
2nd locus specific primer 20wq: 5'-GGAATGATTATTAGGGCAAAACCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11