Genotyping and primer details


Genotyping and primer details for SALK_121158c - At1g30500
Line ID SALK_121158c
Locus At1g30500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:10805276 of line SALK_121158c Chr1:10805276
   
Insertion allele amplicon LBb3 - 96by
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 96by: 5'-TTTCTGGTTTTGCTCTCTCTCTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:10805874
   
wt amplicon (over InsPos) 01dd - 96by
wt amplicon start Chr1:10805074
wt amplicon end Chr1:10805874
wt amplicon size 801 bp
Locus specific primer 96by: 5'-TTTCTGGTTTTGCTCTCTCTCTTC-3'
2nd locus specific primer 01dd: 5'-TTAACAAAAACAGGTTCCTCGACT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11