Genotyping and primer details


Genotyping and primer details for SALK_121910c - At3g62310
Line ID SALK_121910c
Locus At3g62310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:23057852 of line SALK_121910c Chr3:23057852
   
Insertion allele amplicon LBb3 - 27ht
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 27ht: 5'-AAAAAGCAGCAGATGAAGCTAAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:19400783
   
wt amplicon (over InsPos) 07ls - 27ht
wt amplicon start Chr2:19400783
wt amplicon end Chr3:23057503
wt amplicon size 867 bp
Locus specific primer 27ht: 5'-AAAAAGCAGCAGATGAAGCTAAAG-3'
2nd locus specific primer 07ls: 5'-ATAAGATTCTCCTTCATTTCCGGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11