Sequences from confirmation amplicon


Confirmation sequences for line SALK_121910c and hit At3g62310 are shown.
Line ID SALK_121910c
Locus At3g62310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:23057852
Primers used for PCR 27ht - LBb3, show primer details
   
Sequence Name 07-K113843-SALK_121910C-27ht
GenBank Accession LN484464 [GenBank]
Sequencing primer gene specific primer, 27ht: 5'-AAAAAGCAGCAGATGAAGCTAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K113843-SALK_121910C-27ht
GCTTTTCAGCAAACAGTAAGTGATTATCCCTTTATCCTTCCTCATTGGTTTATAGTTGCT
TTAGACAAATGTAATGACTGTAACTAACTTGATCAATGTGAATTCTCGCAGATGAGGATC
CAAATTGGTGCTATGAAAACTTCATCAACAATAGAGCGATGAAATCTGCAGACAATGTTA
GACAGCAGCTAGTCCGGATAATGTCAAGATTCAACCTGAAGATGTGCAGCACTGATTTTA
ACAGCCGCGACTACTACATCAACATAAGGAAGGCCATGCTTGCCGGTTATTTCATGCAAG
TGGCTCACTTAGAACGTACTGGCCATTACTTGACCGTCAAAGACAACCAAGTATGTCAAA
ACAAATTCAGTTTAGGTTGTTTCCATTTAAAATGTTTCTCTTTGTTCTAATCTGATGGTT
ATATTGGCAGGTGGTTCATTTGCATCCATCCAATTGCTTGGTGGATATATTGTGGTGTAA
ACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGGT
GGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTG
AGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGAT
GGTGGTTCC
   
Sequence Name 03-K113842-SALK_121910C-R210
GenBank Accession LN484342 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K113842-SALK_121910C-R210
AAATGAACCACCTGCCAATATAACCATCAGATTAGAACAAAGAGAAACATTTTAAATGGA
AACAACCTAAACTGAATTTGTTTTGACATACTTGGTTGTCTTTGACGGTCAAGTAATGGC
CAGTACGTTCTAAGTGAGCCACTTGCATGAAATAACCGGCAAGCATGGCCTTCCTTATGT
TGATGTAGTAGTCGCGGCTGTTAAAATCAGTGCTGCACATCTTCAGGTTGAATCTTGACA
TTATCCGGACTAGCTGCTGTCTAACATTGTCTGCAGATTTCATCGCTCTATTGTTGATGA
AGTTTTCATAGCACCAATTTGGATCCTCATCTGCGAGAATTCACATTGATCAAGTTAGTT
ACAGTCATTACATTTGTCTAAAGCAACTATAAACCAATGAGGAAGATAAAGGGATAATCA
CTTACTGTTTTGCTTGAAAGCGTGGTAGACATTCAACAATGTGAGGTGATCTCCCTCAAT
GTGTCCAAACCTAGCTTTAGCTTC

Last Updated on 06.12.2018 16:11