Genotyping and primer details


Genotyping and primer details for SALK_121927c - At3g62730
Line ID SALK_121927c
Locus At3g62730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:23208523 of line SALK_121927c Chr3:23208523
   
Insertion allele amplicon LBb3 - 87cs
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 87cs: 5'-GTCCGCTAATAAATCTGACGAGAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:23209011
   
wt amplicon (over InsPos) 28dz - 87cs
wt amplicon start Chr3:23208269
wt amplicon end Chr3:23209011
wt amplicon size 743 bp
Locus specific primer 87cs: 5'-GTCCGCTAATAAATCTGACGAGAG-3'
2nd locus specific primer 28dz: 5'-TACCGAGTTCATTACGGAGGTTAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11