Genotyping and primer details


Genotyping and primer details for SALK_121983c - At2g25180
Line ID SALK_121983c
Locus At2g25180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:10726384 of line SALK_121983c Chr2:10726384
   
Insertion allele amplicon LBb3 - BI38
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer BI38: 5'-TCAAAACAACACACACAAAACAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:10725816
   
wt amplicon (over InsPos) BI39 - BI38
wt amplicon start Chr2:10725816
wt amplicon end Chr2:10726427
wt amplicon size 612 bp
Locus specific primer BI38: 5'-TCAAAACAACACACACAAAACAGA-3'
2nd locus specific primer BI39: 5'-TCTTGAAATGGACCTACCTGTCAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11