Genotyping and primer details


Genotyping and primer details for SALK_122275 - At3g10915
Line ID SALK_122275
Locus At3g10915 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:3417860 of line SALK_122275 Chr3:3417860
   
Insertion allele amplicon R204 - 57aq
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer 57aq: 5'-ACAAACCAATCGATCAAACACATA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3418386
   
wt amplicon (over InsPos) 05bp - 57aq
wt amplicon start Chr3:3417598
wt amplicon end Chr3:3418386
wt amplicon size 789 bp
Locus specific primer 57aq: 5'-ACAAACCAATCGATCAAACACATA-3'
2nd locus specific primer 05bp: 5'-ACGATCTCAGTACAATGGTTTGTG-3'
   
wt amplicon (over InsPos) 01tu - 57aq
wt amplicon start Chr3:3417598
wt amplicon end Chr3:3418386
wt amplicon size 789 bp
Locus specific primer 57aq: 5'-ACAAACCAATCGATCAAACACATA-3'
2nd locus specific primer 01tu: 5'-ACGATCTCAGTACAATGGTTTGTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11