Sequences from confirmation amplicon


Confirmation sequences for line SALK_122275 and hit At3g10915 are shown.
Line ID SALK_122275
Locus At3g10915 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3417860
Primers used for PCR 57aq - R204, show primer details
   
Sequence Name 53-K107035-SALK_122275C-R210
GenBank Accession LN485571 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K107035-SALK_122275C-R210
CGTCATTTGTTTACCCACAATATATCCTGGACATATTACGGCCCATAAATAAAAATCACA
TGTCTACGTGTCAAAACAGTCACTTTTCTCTATACATGACAGGCAGTGATATTATCATAA
CCCTCCACTTATACACATGTTCATTTTTGGCGACCACTAGAAGGAGAAACAAATCTTCAT
TCCAACAATGGCGATTCGTTGTGTAGCGAGTAGAAAAACCCTAGCCGGCTTGAAGGAGAC
ATCATCGAGGCTATTGAGGATCAGAGGGATTCAGACTTTTACGCTTCCTGATCTTCCTTA
CGATTATGGCGCATTGGAACCGGCCATTAGTGGAGAGATCATGCAGATTCATCACCAGAA
GCATCACCAGGCTTATGTTACTAATTACAATAATGCTCTTGAGCAGCTTGATCAAGCTGT
GAACAAGGGAGATGCTTCCACTGTTGTTAAGTTGCAGAGCGCCATCAAATTCAACGGCGG
AGGTGTGTTTGTGTGTCTGACTCTCTTTTTTTTTTTTTTTAACAACCCCAATTTCTATGT
GTTTAACCAATGGGGTTT
   
Sequence Name 61-K107036-SALK_122275C-57aq
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 57aq: 5'-ACAAACCAATCGATCAAACACATA-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K107036-SALK_122275C-57aq
AAAAAAAGAAGTCAAACCCCCAACCACCCCTCCCCCGTTTAAATTTGAGGGCCCTCTGCA
CCTTAACAACAGGGAAACCTTCCCCCTTGTTCACAGTTTGATCAACCTGCCCAAAACCTT
TTTTGTAATTAGAAACAAAACCCGGGGAATCCTTCGGGGAAAAAATCTGCATAATCCCCC
CCCAAAGGGCCGGTTCCAATGCCCCATAATCGTAGGGAAAATCAGGAACCGAAAAATTCT
AAACCCCTCTAACCCTCAAAACCCCCAATAATTTCCCCTTCAACCCGGCTAGGGTTTTTC
TCCCCCCTACACAACAAACCCCCTTTGTGGAAAAAAAAATTTTTTTCCCCTTCTAGGGGC
CCCCAAAAATAAACTTGTGTATAAGGGGGGGGTTATAAAAATACCCCCCCCTGCCATGTA
AAAAAAAAAGTGACTGTTTTAACCCTAAAACTTGTATTTTTTTTTTTGGGGCCGAAATAT
GCCCAGAATATTTTGGGGGGAAAACAATTTGCCCCTTAAACAACTTAAAAACCCTTTGGG
AACTTTTTTAATGTACGGGGGGGGTTTTTTTTTTCCCCAGGGAAACGGGCAACAGTTAAT
TGCCCTTCCCCCCCGGGCCCTAAAAAATTTCCA

Last Updated on 06.12.2018 16:11