Genotyping and primer details


Genotyping and primer details for SALK_122484c - At1g48450
Line ID SALK_122484c
Locus At1g48450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:17910382 of line SALK_122484c Chr1:17910382
   
Insertion allele amplicon LBb3 - 48fx
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 48fx: 5'-TTCTCCTAAAGACCCAAGAAACAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:17909585
   
wt amplicon (over InsPos) 04hv - 48fx
wt amplicon start Chr1:17909585
wt amplicon end Chr1:17910671
wt amplicon size 1087 bp
Locus specific primer 48fx: 5'-TTCTCCTAAAGACCCAAGAAACAA-3'
2nd locus specific primer 04hv: 5'-CCTAATTGTCTTGATGGGTCGTAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11