Genotyping and primer details


Genotyping and primer details for SALK_122584c - At1g69230
Line ID SALK_122584c
Locus At1g69230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:26026726 of line SALK_122584c Chr1:26026726
   
Insertion allele amplicon LBb3 - 23nt
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 23nt: 5'-AACAATTGAAGCACATATGCGTAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:26026406
   
wt amplicon (over InsPos) 09or - 23nt
wt amplicon start Chr1:26026406
wt amplicon end Chr1:26027324
wt amplicon size 919 bp
Locus specific primer 23nt: 5'-AACAATTGAAGCACATATGCGTAG-3'
2nd locus specific primer 09or: 5'-AATTCATGTTTTGGTTTTGTTTCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11