Sequences from confirmation amplicon


Confirmation sequences for line SALK_122584c and hit At1g69230 are shown.
Line ID SALK_122584c
Locus At1g69230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26026726
Primers used for PCR 23nt - LBb3, show primer details
   
Sequence Name 73-K114703-SALK_122584C-23nt
GenBank Accession LN486084 [GenBank]
Sequencing primer gene specific primer, 23nt: 5'-AACAATTGAAGCACATATGCGTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K114703-SALK_122584C-23nt
ATTTATTTTTTGCTGGGAACAGAGGTTTCACAAAATGATAGAAGTGTGACGTGGGATAAA
GAAGATAGAGTGACCAAGTTGGCCTCAGTAGCTAGTTGCTACCACCACCAAAGAGGTAAT
CAAGAGACGAGCCACCTCCTGGAGCTGAGTGAACCTTGGTCGATGGTCGGTCCTGCGAAC
AACAACCAAACAAGATGTTTTAGTAACTGTACCATAAGTTTTAGACGATTATTGCGTTGT
TAGCACAAAGCAAAACCCATCAGTCTATTTGATCTAAGAAAACAAATTGACGCTTAGACA
ACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTG
AGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGT
CCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAATAAGGCCA
AAA
   
Sequence Name 64-K114702-SALK_122584C-R210
GenBank Accession LN485853 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K114702-SALK_122584C-R210
TTTGTTTTCTTAGATCAATAGACTGATGGGTTTTGCTTTGTGCTAACAACGCAATAATCG
TCTAAAACTTATGGTACAGTTACTAAAACATCTTGTTTGGTTGTTGTTCGCAGGACCGAC
CATCGACCAAGGTTCACTCAGCTCCAGGAGGTGGCTCGTCTCTTGATTACCTCTTTGGTG
GTGGTAGCAACTAGCTACTGAGGCCAACTTGGTCACTCTATCTTCTTTATCCCACGTCAC
ACTTCTATCATTTTGTGAAACCTCTTGTTCCCTAGCAAAAAATAAAATCTGTATAATCTC
TAAATTTCTACGCATATGTGCTTCAATTGT

Last Updated on 06.12.2018 16:11