Genotyping and primer details


Genotyping and primer details for SALK_122849 - At1g02280
Line ID SALK_122849
Locus At1g02280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:449073 of line SALK_122849 Chr1:449073
   
Insertion allele amplicon LBb3 - 36mv
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 36mv: 5'-TGGATGTGTATAGAGTCGATGAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:449442
   
wt amplicon (over InsPos) 16oj - 36mv
wt amplicon start Chr1:448665
wt amplicon end Chr1:449442
wt amplicon size 778 bp
Locus specific primer 36mv: 5'-TGGATGTGTATAGAGTCGATGAGC-3'
2nd locus specific primer 16oj: 5'-TTAAAGTGGCTTTCCACTTGTCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11