Genotyping and primer details


Genotyping and primer details for SALK_122890c - At3g21700
Line ID SALK_122890c
Locus At3g21700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:7646095 of line SALK_122890c Chr3:7646095
   
Insertion allele amplicon LBb3 - 30hd
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 30hd: 5'-CCAGGTCTAATCTTGTTGTGTGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:7645505
   
wt amplicon (over InsPos) 21ir - 30hd
wt amplicon start Chr3:7645505
wt amplicon end Chr3:7646325
wt amplicon size 821 bp
Locus specific primer 30hd: 5'-CCAGGTCTAATCTTGTTGTGTGAA-3'
2nd locus specific primer 21ir: 5'-CATCTCTCCATTTCGCTTAAATTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11