Genotyping and primer details


Genotyping and primer details for SALK_122971c - At5g61710
Line ID SALK_122971c
Locus At5g61710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:24799926 of line SALK_122971c Chr5:24799926
   
Insertion allele amplicon LBb3 - 49sf
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 49sf: 5'-ATCATATCATCTCCTCCTTGGACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:24800403
   
wt amplicon (over InsPos) 04tp - 49sf
wt amplicon start Chr5:24799380
wt amplicon end Chr5:24800403
wt amplicon size 1024 bp
Locus specific primer 49sf: 5'-ATCATATCATCTCCTCCTTGGACA-3'
2nd locus specific primer 04tp: 5'-TTTCTTGTATCATATCCGCAAGGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11