Genotyping and primer details


Genotyping and primer details for SALK_123009c - At1g18710
Line ID SALK_123009c
Locus At1g18710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6452209 of line SALK_123009c Chr1:6452209
   
Insertion allele amplicon LBb3 - 55dl
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 55dl: 5'-CCCTATTTGTTGCTGAAAGTTCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6451439
   
wt amplicon (over InsPos) 25lt - 55dl
wt amplicon start Chr1:6451439
wt amplicon end Chr1:6452518
wt amplicon size 1080 bp
Locus specific primer 55dl: 5'-CCCTATTTGTTGCTGAAAGTTCTC-3'
2nd locus specific primer 25lt: 5'-GAGACAAGAGTTCCAATGGTTCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11