Genotyping and primer details


Genotyping and primer details for SALK_123093c - At5g04990
Line ID SALK_123093c
Locus At5g04990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:1472014 of line SALK_123093c Chr5:1472014
   
Insertion allele amplicon LBb3 - 64vi
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 64vi: 5'-CAATGTTTTCCTCTGAAAGGTAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:1472777
   
wt amplicon (over InsPos) 73vw - 64vi
wt amplicon start Chr5:1471537
wt amplicon end Chr5:1472777
wt amplicon size 1241 bp
Locus specific primer 64vi: 5'-CAATGTTTTCCTCTGAAAGGTAGC-3'
2nd locus specific primer 73vw: 5'-GCAACCAAATAAACCAAAAGGTAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11