Genotyping and primer details


Genotyping and primer details for SALK_123621c - At4g13660
Line ID SALK_123621c
Locus At4g13660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:7947732 of line SALK_123621c Chr4:7947732
   
Insertion allele amplicon LBb3 - 41qp
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 41qp: 5'-CCTTTTTGGCTTACCAATAACAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:7946997
   
wt amplicon (over InsPos) 11rz - 41qp
wt amplicon start Chr4:7946997
wt amplicon end Chr4:7948168
wt amplicon size 1172 bp
Locus specific primer 41qp: 5'-CCTTTTTGGCTTACCAATAACAAA-3'
2nd locus specific primer 11rz: 5'-TTCATGATCTGTGAGACAACCTTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11