Genotyping and primer details


Genotyping and primer details for SALK_123960c - At4g38550
Line ID SALK_123960c
Locus At4g38550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:18026061 of line SALK_123960c Chr4:18026061
   
Insertion allele amplicon LBb3 - 69ns
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 69ns: 5'-TAGACTTTTCCAATTGCCATTTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:18025573
   
wt amplicon (over InsPos) 03ph - 69ns
wt amplicon start Chr4:18025573
wt amplicon end Chr4:18026662
wt amplicon size 1090 bp
Locus specific primer 69ns: 5'-TAGACTTTTCCAATTGCCATTTTC-3'
2nd locus specific primer 03ph: 5'-CTTCGGGTATCTGCTTCATACATT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11