Genotyping and primer details


Genotyping and primer details for SALK_124375c - At1g05340
Line ID SALK_124375c
Locus At1g05340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:1559367 of line SALK_124375c Chr1:1559367
   
Insertion allele amplicon LBb3 - 15mh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 15mh: 5'-GATGTTTGAGAAGTCAGAACACCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:1560032
   
wt amplicon (over InsPos) 08nl - 15mh
wt amplicon start Chr1:1559044
wt amplicon end Chr1:1560032
wt amplicon size 989 bp
Locus specific primer 15mh: 5'-GATGTTTGAGAAGTCAGAACACCA-3'
2nd locus specific primer 08nl: 5'-TCGTCATAAATAGACCATCCCAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11