Genotyping and primer details


Genotyping and primer details for SALK_124643c - At1g48450
Line ID SALK_124643c
Locus At1g48450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:17909116 of line SALK_124643c Chr1:17909116
   
Insertion allele amplicon LBb3 - 30fx
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 30fx: 5'-TTCAGAAGTTCATGGATGCTAATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:17909521
   
wt amplicon (over InsPos) 03ho - 30fx
wt amplicon start Chr1:17908840
wt amplicon end Chr1:17909521
wt amplicon size 682 bp
Locus specific primer 30fx: 5'-TTCAGAAGTTCATGGATGCTAATG-3'
2nd locus specific primer 03ho: 5'-AATCCCTTGAAGCTGATCTTGATA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11