Sequences from confirmation amplicon


Confirmation sequences for line SALK_124643c and hit At1g48450 are shown.
Line ID SALK_124643c
Locus At1g48450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:17909116
Primers used for PCR 30fx - LBb3, show primer details
   
Sequence Name 27-K112064-SALK_124643C-30fx
GenBank Accession LN485060 [GenBank]
Sequencing primer gene specific primer, 30fx: 5'-TTCAGAAGTTCATGGATGCTAATG-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K112064-SALK_124643C-30fx
CGGATCCATCTGGTCGAGTTGATACTTGGCCAACTCTAGATGGGGAACTTGAGAGACTTC
ACTCGCCTGAAGTTTATGAGATGATTCAGAATCATCTTTCCATCATTCTTAAAAACCGCA
CTGATGATCTGACAGCAGTTGCGCAGATAAGCAAACTTGGAGTTGGACAGGTATATGCTG
CTTCGGTGATGTATGGGTATTTCCTGAAGAGGATCGATCAAAGGTTTCAGCTTGAGAAGA
CGATGAGGATTCTCCCAGGTGGGTCAGATGAAGGTGAGACTAGCATTGAGCAAGCAGGCC
GAGATGTAGAGAGAAACTTCTATGAAGAAGCAGAAGAGACTTATCAGGCTGTTTCCTCAA
CTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGA
GACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTC
CACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 11-K112049-SALK_124643C-R210
GenBank Accession LN484583 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K112049-SALK_124643C-R210
GACGCCTGATAGTCTCTTCGCTTCTTCATAGAAGTTTCTCTCTCATCTCGGCCTGCTTGC
TCAATGCTAGTCTCACCTTCATCTGACCCACCTGGGAGAATCCTCATCGTCTTCTCAAGC
TGAAACCTTTGATCGATCCTCTTCAGGAAATACCCATACATCACCGAAGCAGCATATACC
TGTCCAACTCCAAGTTTGCTTATCTGCGCAACTGCTGTCAGATCATCAGTGCGGTTTTTA
AGAATGATGGAAAGATGATTCTGAATCATCTCATAAACTTCAGGCGAGTGAAGTCTCTCA
AGTTCCCCATCTAGAGTTGGCCAAGTATCAACTCGACCAGATGGATCCGCAGATGAAGAG
GTTATGGATGGTACAAGTGTAACATTAGCATCCATGAACCTTCTGA

Last Updated on 06.12.2018 16:11