Genotyping and primer details


Genotyping and primer details for SALK_125328c - At1g04830
Line ID SALK_125328c
Locus At1g04830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:1359419 of line SALK_125328c Chr1:1359419
   
Insertion allele amplicon LBb3 - 26md
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 26md: 5'-TGTGTAAATCATTACAGGGTGGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:1358771
   
wt amplicon (over InsPos) 14nv - 26md
wt amplicon start Chr1:1358771
wt amplicon end Chr1:1359878
wt amplicon size 1108 bp
Locus specific primer 26md: 5'-TGTGTAAATCATTACAGGGTGGAA-3'
2nd locus specific primer 14nv: 5'-TTCTTTTGCTTTGTTGAATTGTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11