Genotyping and primer details


Genotyping and primer details for SALK_125922c - At2g44750
Line ID SALK_125922c
Locus At2g44750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:18452641 of line SALK_125922c Chr2:18452641
   
Insertion allele amplicon LBb3 - 49wu
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 49wu: 5'-GATCTAACATGCTTTTTCTCCAGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:18452064
   
wt amplicon (over InsPos) 32xo - 49wu
wt amplicon start Chr2:18452064
wt amplicon end Chr2:18453294
wt amplicon size 1231 bp
Locus specific primer 49wu: 5'-GATCTAACATGCTTTTTCTCCAGG-3'
2nd locus specific primer 32xo: 5'-AGTAAATGCAACTCCACTCTACGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11