Genotyping and primer details

Genotyping and primer details for SALK_126363c - At5g22790
Line ID SALK_126363c
Locus At5g22790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:7601100 of line SALK_126363c Chr5:7601100
Insertion allele amplicon LBb3 - 19ck
Locus specific primer 19ck: 5'-TGAAGCTTTTAAGCATCTGGGTAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:7601637
wt amplicon (over InsPos) 26dn - 19ck
wt amplicon start Chr5:7600984
wt amplicon end Chr5:7601637
wt amplicon size 654 bp
Locus specific primer 19ck: 5'-TGAAGCTTTTAAGCATCTGGGTAT-3'
2nd locus specific primer 26dn: 5'-TTTTTCTCAAAAGCAAAAACCTGT-3'
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11