Genotyping and primer details


Genotyping and primer details for SALK_126628c - At2g20680
Line ID SALK_126628c
Locus At2g20680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:8922657 of line SALK_126628c Chr2:8922657
   
Insertion allele amplicon LBb3 - 16cz
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 16cz: 5'-AGGTTGCTTCTGAGCTTAGTGAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:8921755
   
wt amplicon (over InsPos) 29dz - 16cz
wt amplicon start Chr2:8921755
wt amplicon end Chr2:8922731
wt amplicon size 977 bp
Locus specific primer 16cz: 5'-AGGTTGCTTCTGAGCTTAGTGAAT-3'
2nd locus specific primer 29dz: 5'-CATCTTCAATGTGAGATAGCATCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11