Sequences from confirmation amplicon


Confirmation sequences for line SALK_126628c and hit At2g20680 are shown.
Line ID SALK_126628c
Locus At2g20680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8922657
Primers used for PCR 16cz - LBb3, show primer details
   
Sequence Name 81-K109033-SALK_126628C-16cz
GenBank Accession LN486231 [GenBank]
Sequencing primer gene specific primer, 16cz: 5'-AGGTTGCTTCTGAGCTTAGTGAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K109033-SALK_126628C-16cz
GTCATTGGGCATGGCACGAAGGTGTAGGCCTCAGCTCATCCATGCCTTTATGGTTCTTTG
ACCCATCTATCCGCAGATACTTCAAGACTTATCTCACGGTATGTTTTTTTTAATCTTATT
TCTTTTCTATGTGAAGATGGATGTGTTAGTCTTGATCATCATAGTTGAATTAGATACTGA
ATTCAGAAACTTTGTTCTCGCTTGACCGGTAGACTTGGATAAGTTTCCGCACAAAAGCTT
TTCTTCACTTATAGGCTTTGATTTTTCATGGTTGTGTAATTGATGTTGAGCTTGTAATTA
CTCTCATTTTCATATGCCAGGTGCTGCTGACCCGTAAAAACTCTTTAACCGGGATTGAGT
ACAGAAACGACCCAACAATTTTTGCATGGGAGTTGATTAACGAGCCTCGCTGCATGTCAG
ATGTCTCTGGGGAT
   
Sequence Name 65-K109032-SALK_126628C-R210
GenBank Accession LN485867 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K109032-SALK_126628C-R210
GTCATTTGTTTCCCAAAGTTTCAAACTTTGTGGTTTACAACAAGATTTCCACACCTTTGT
TCACACAGTTCTTAACAGAGGCTTACCAGTGATCAGGGTAGATATGAACCGAGGCAAAAT
CGATGTTTGGCGAATCAGAGTTTCGAACAAAATCTGACCCAAGCTCAGAGGCCCACCGTT
CGGGGTTAACTGTCAGCTTTTTTGGGCTACTGGGACCGTAGAACCCTTCCAAGCCAACAG
TTAGAAGATGCTTGTTGTCAATTGATTTAATGAATGCTGTCATTTCGTTAATCCAGTCCT
TTAAATCCACATACCAGAAAAAGAAACCACAAGAGTAACATTACTAATTTTCTCAACAAT
TACTAATGATCCAATGCTGGACAAGAAAACCACCAAAATGAAGGAGAACTCACTTGTAGA
GTATCCCCAGAGACATCAGACATGCAGCGAGGCTCGTTTATCAACTCCCATGCAAAAATT
GTTGGGTCGTTTCTGTACTCAATCCCGGTTAAAGAGTTTTTACGGGTCAGCAGCACCTGG
CATATGAAAATGAGAGTAATTACAAGCTCAACCTCCAATTACACAACCCTGAAAAATCAA
AGCCTATAAGTGAAGAAAAGCTTTTGTGCGGAAACTTATCCCAGTCTACCGGCCCAGCGA
GAACAAAGTTTCTGGAATCAGTATCTAATTCAACTATGATGA

Last Updated on 06.12.2018 16:11