Genotyping and primer details


Genotyping and primer details for SALK_126948c - At1g78510
Line ID SALK_126948c
Locus At1g78510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:29537058 of line SALK_126948c Chr1:29537058
   
Insertion allele amplicon LBb3 - 97by
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 97by: 5'-AAAAACTCGATCGGCTGATACTTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:29537376
   
wt amplicon (over InsPos) 13dt - 97by
wt amplicon start Chr1:29536716
wt amplicon end Chr1:29537376
wt amplicon size 661 bp
Locus specific primer 97by: 5'-AAAAACTCGATCGGCTGATACTTA-3'
2nd locus specific primer 13dt: 5'-CAAAGTCTGCAGATCAACAGCTAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11