Genotyping and primer details


Genotyping and primer details for SALK_127167c - At1g24150
Line ID SALK_127167c
Locus At1g24150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:8549581 of line SALK_127167c Chr1:8549581
   
Insertion allele amplicon LBb3 - 28di
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 28di: 5'-AGTCTTGACATGAGGAGGATGAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:8550210
   
wt amplicon (over InsPos) 01ev - 28di
wt amplicon start Chr1:8549136
wt amplicon end Chr1:8550210
wt amplicon size 1075 bp
Locus specific primer 28di: 5'-AGTCTTGACATGAGGAGGATGAGT-3'
2nd locus specific primer 01ev: 5'-AGCACTGAAGTCTTTGAGAGGAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11