Genotyping and primer details


Genotyping and primer details for SALK_127263c - At3g24110
Line ID SALK_127263c
Locus At3g24110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:8705242 of line SALK_127263c Chr3:8705242
   
Insertion allele amplicon LBb3 - 28sm
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 28sm: 5'-TGTTTCCTTTAACCTTTGCCTCTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:8705757
   
wt amplicon (over InsPos) 10tl - 28sm
wt amplicon start Chr3:8704632
wt amplicon end Chr3:8705757
wt amplicon size 1126 bp
Locus specific primer 28sm: 5'-TGTTTCCTTTAACCTTTGCCTCTA-3'
2nd locus specific primer 10tl: 5'-TACCAAATCTCATGTTGGTGACAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11